The New York University Genetic Analysis Center (NYU GAC) is a facility that is dedicated to delivering high-quality DNA sequencing and genotyping services to the scientific community in a short turnaround time (24-48 hrs from receipt). The facility operates a 96-Well GeneAmp® PCR System 9700 and an ABI 3100 capillary sequencer that produces accurate, high quality reads of between 600-800 bp. The NYU GAC sequences purified PCR products, double stranded plasmid DNA, BAC's and Cosmids using BigDye® Terminator v3.1 Cycle Sequencing chemistry. For recommended purification procedures and required template preparation, please consult the Template Preparation Instructions. Researchers must fill out the Sequencing Submission Form and send it electronically to nyugac@nyu.edu, as well as print out a hard copy to be delivered with the samples to be sequenced. Template and primers can be dropped off at Brown 957, Mon-Fri 9:00am-5:00pm or mailed to the address above. If the door is closed put samples and order form in an envelope and drop in mail box.
Facility Location:
New York University
Genetic Analysis Facility
Department of Biology
Brown Building, Room 957
100 Washington Square East
New York, NY 10003
Facility Manager (to whom all correspondence should be addressed):
Locke Uppendahl
Department of Biology
New York University
Brown Building, Room 957
100 Washington Square East
New York, NY 10003
Tel: (212) 998-8703
Email: nyugac@nyu.edu
Facility Director:
David H. A. Fitch
Associate Professor
Department of Biology
New York University
Main Building, Room 1009
100 Washington Square East
New York, NY 10003
Email: david.fitch@nyu.edu
Sequencing Charges:
$10/sample or $900/96 samples (BigDye sequencing rxn + ABI 3100 run)
$6/sample or $540/96 samples (ABI 3100 run)
Resource Links:
NYU GAC Equipment and Chemistry:
ABI PRISM® 3100 Genetic Analyzer
96-Well GeneAmp® PCR System 9700
BigDye® Terminator v3.1 Cycle Sequencing Kit
Electropherogram and sequence viewing software:
ABI PRISM® 3100 Genetic Analyzer Conversion Utilities (Windows & MAC OS X)
Finch TV (Windows & MAC OS X)
Chromas Lite (Windows)
Edit View (MAC OS 9)
4Peaks (MAC OS X)
Sequencher (Demo) (Windows & MAC)
Sequence alignment software and sites:
ClustalX (Windows & MAC)
EXPASY SIM Alignment Tool
Primer Design Sites and Software:
Net Primer
Primer 3
Amplify 3 (MAC)
IDT PrimerQuest
Other Useful Sites:
Applied Biosystems Inc.
National Center for Biotechnology Information (NCBI)
